| Abstrak/Abstract |
Conjugated linoelic acid (CLA) is bioactive compound that can be synthesized by probiotic.
Lactobacillus casei strain AP and AG were isolated from Indonesian infant and had a
potential as probiotic culture. The objective of this study was to detect and measure level
transcription of the genes responsible for CLA synthesis in Lactobacillus casei. The gene
detection was performed by amplification of parsial genom in conserve location of clahy,
cla-dh, and cla-dc genes from Lactobacillus plantarum AKU1009a. Bacterial culture was
grown in media with and without addition of linoleic acid (0.4 mg/ml). The transcription of cla-hy, cla-dh, and cla-dc homolog genes were analyzed by quantitative Real
TimePolymerase Chain Reaction (qRT-PCR). Amplification products in L. casei strain AG
was 143, 206, and 148 bp respectively. These sequences were identified as parsial cla-hy,
cla-dh, and cla-dc homolog genes. The same primer did not amplify cla-hy, cla-dh, and cladc
genes in L. casei strain AP. Level of transcription of cla-hy, cla-dh, and cla-dc homolog
gene in L. casei strain AG grown in media containing linoleic acid 0.4 mg/ was 0.74; 0.69;
and 1.43 fold change respectively, and this was not significant (P>0.05). The transcription
of cla-hy, cla-dh, and cla-dc homolog genes in L. casei strain AG was not induced by
addition of linoleic acid at 0.4 mg/ml. |
