Karya
Judul/Title Detection and Identification of Banana-associated Phytoplasma Using Nested-PCR Method
Penulis/Author SAURMA MONA ASTRID S (1) ; Prof. Dr. Tri Joko, S.P., M.Sc. (2); Prof. Dr. Ir. Siti Subandiyah, M.Agr.Sc. (3)
Tanggal/Date 2019
Kata Kunci/Keyword
Abstrak/Abstract Phytoplasma is known to be associated with plant diseases in about 300 plant species from various families.I nformation on the presence of resence of phytoplasma in bananas as one of the pathogens that can cause disease in bananas inI ndonesia has never been reported. This research was conducted with the aim to detect the presence of bananap hytoplasma by the nested-PCR method and to identify phytoplasma obtained based on the sequence analysis of the1 6S rRNA gene. Standard PCR was carried out using P1/P7 primary pairs, followed by nested-PCR using a pair ofR 16F2n/R16R2m23SR primers separately that could amplify the target 16S rRNA genes in a row at 1600 bp. BLASTa nalysis shows that the results of phylogenetic analysis of banana phytoplasmic nucleotide cv. manggala fromT asikmalaya and cv. Raja nangka from Banjar has a genetic relationship that is closer to lethal wilt oil palm Phytoplasma( CandidatusPhytoplasma asteris). This phytoplasma belongs to the 16SrI-B group (aster yellows)
Rumpun Ilmu Ilmu Hama dan Penyakit Tanaman
Bahasa Asli/Original Language English
Level Nasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1Mona-JPTI.pdf[PAK] Full Dokumen
2Detection and Identification of Banana-associated Phytoplasma____compressed.pdf[PAK] Full Dokumen
3Sertifikat Akreditasi JPTI peringkat 2 tahun 2017 s_d_ 2021.pdfDokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)