Detection and Identification of Adherence Genes of Intestinal-origin Lactobacillus and Pediococcus Strains Grown on Gastric Mucin in vitro
Penulis/Author
Prof. Widodo, S.P., M.Sc. Ph.D. (1); Prof. drh. Widya Asmara, SU., Ph.D. (2)
Tanggal/Date
2015
Kata Kunci/Keyword
Abstrak/Abstract
One of primary selection criteria for potential probiotics is the ability to adhere on the host gastrointestinal tract. This study evaluate the in vitro adhesion ability on gastric mucin of two Lactobacillus casei strains (AP and AG) and Pediococcus acidilactici strains (BE and BK), and identify the corresponding genes responsible for the adherence. Adhesion assays were performed in 96-well polystyrene microtiter plates using gastric mucin from porcine stomach as the matrix. In vitro study on gastric mucin revealed that lactobacilli had higher adherence ability compared to pediococci strains. The potential adherence genes were investigated using Polymerase Chain Reaction (PCR) technology. Using specific primers, PCR studies amplified 150 base pairs of potential mub gene and 161 base pairs of potential ef-Tu gene, but no amplified bands for potential map and bac genes was obtained. Sequence comparison showed that the 150 and 161 amplified base pa
