| Abstrak/Abstract |
Yogyakarta is one of the cities in Indonesia that regularly experiencing dengue outbreaks. In an effort to tackle the disease, the city was selected as a site for the Applying Wolbachia to Eliminate Dengue (AWED) study in 2017. Prior to the Wolbachia-infected Aedes aegypti release trial in the city, we conducted a study on dengue epidemiology, entomology, and virology to gain insight on the dynamics of the disease and provide baseline data in the city. Dengue epidemiological data was compiled, and dengue patient samples were tested for dengue infection using dengue virus (DENV) Non-Structural protein 1 (NS1) antigen, IgM/IgG serology, and RT-PCR detections. The city’s demographic and meteorological data were recorded. Ae. aegypti mosquito samples were caught from the field using BG-Sentinel trap. DENV detection and serotyping were conducted in both patient and mosquito samples and Envelope gene sequencing was used to determine the phylogeny and genotypes of circulating DENV. Our study reveals that annual dengue incidence peaked during the early months of the years, with a full-year dengue epidemic occurred in 2016-2017. Most cases occurred in children. Serotyping revealed the presence of all four DENV serotypes with DENV-3 as the predominant serotype (88 or 44.67%), followed by DENV-1 (87 or 44.16%), DENV-4 (12 or 6.09%) and DENV-2 (10 or 5.08%). Yogyakarta DENV-1 isolates consisted of Genotype I and IV while DENV-2, DENV-3 and DENV-4 belong to the Cosmopolitan genotype, Genotype I, and Genotype II, respectively. Yogyakarta DENV isolates were closely related to Indonesian strains from its neighboring Javan cities, plausibly through endemic circulation. Our study provides comprehensive information on the epidemiology and viral characteristics in Yogyakarta, which are useful as baseline data for AWED study and DENV surveillance in the city. |
