DECREASE OF BLOOD GLUCOSE AND TRIGLYSERIDA CORRELATED WITH INCREASE OF AMPK-?2 EXPRESSION IN SKELETAL MUSCLE OF DIABETIC RATS AFTER SUPPLEMENTATION OF SYNBIOTIC
Penulis/Author
Prof. Dr. Dra. Sunarti, M.Kes. (1); Dr. Rio Jati Kusuma, S.Gz., MS. (2); NENI OKTIYANI (3); TRI SETYAWATI (4)
Tanggal/Date
2017
Kata Kunci/Keyword
Abstrak/Abstract
INTRODUCTION
Adenosine monophosphate-activated protein kinase (AMPK) play an important role in regulating glucose and lipid metabolism. AMPK activity in the liver and muscle tissue can be stimulated by short chain fatty acids (SCFAs) that formed by gut microbiota from complex dietary carbohydrates. The aims of this study were evaluating the correlation of AMPK-?2 expression in skeletal muscle with blood glucose and triglyceride levels in diabetic rats after supplementation of symbiotic.
METHODS
Fifteen male Wistar rats were divided into three groups: 1. normal rat control; 2. diabetic rat control; 3. diabetic rats received symbiotic of E. rectale and D. esculenta. Diabetic induction of rats was performed by intraperitoneally injection of nicotinamide and streptozotocin. After four weeks of the interventions, the blood and skeletal muscles were taken. The AMPK-?2 expression was analyzed with immunohistochemical staining, plasma glucose and triglyceride levels were analyzed using a spectrophotometer.
RESULTS AND DISCUSSION
After intervention, the diabetic rats received symbiotic supplementation had significantly higher the AMPK-?2 expression in the skeletal muscle and lower the blood glucose levels than the diabetic rat control. The triglyceride levels were also reduced after intervention, but it was not statistically significant. There were significant negative correlation between the AMPK-?2 expression with glucose and triglyceride levels. The glucose levels were significantly positively correlated with triglyceride levels. These results suggested that symbiotic induced AMPK-?2 expression in skeletal muscle and reduced blood glucose and triglyceride levels in diabetic rats.
