| Abstrak/Abstract |
Introduction: Fucoidan is a sulfated polysaccharide that has a wide range of bioactivities
including anti-cancer. This polysaccharide commonly is extracted from marine brown
seaweed. There is lack of information on the fucoidan extracted from tropical brown
algae and its anti-cancer activity. Objectives: The objectives of this study were to purify fucoidan from Sargassum sp., Turbinaria sp. and Padina sp., and to evaluate their
cytotoxicity against breast cancer (MCF-7) and colon cancer cells (WiDr). Materials and
Methods: Fucoidan extraction was conducted by using acid extraction method. Purified fucoidans were obtained by DEAE cellulose column chromatography and confirmed
by HPLC and FT-IR spectrometry. The cytotoxicity was evaluated by using the MTT
(3-[4,5-dimethylthiazol-2-yl] -2,5- diphenyltetrazolium bromide) assay. Results: Fucoidan from Sargassum sp. and Turbinaria sp. showed low cytotoxicity with IC50 ranging
between 461-663 µg/mL. Higher cytotoxicity against MCF-7 and WiDr was showed
by fucoidan from Padina sp. with IC
50 of 144 and 118 µg/mL, respectively. While its IC 50 against Vero cells was 501 µg/mL.Standard fucoidan from Fucus vesiculosus exhibited IC
50 of 60, 63 and 211 µg/mL against MCF-7, WiDr and Vero Cells, respectively.
Although the IC50 was higher than that of standard fucoidan, Padina sp. fucoidan showed cytotoxicity comparable with standard fucoidan at concentrations below 100 µg/mL.
Conclusion: These results indicated that Padina sp.fucoidan showed potential selective
cytotoxicity, and promising for the development of an anti-cancer compound. |
