| Abstrak/Abstract |
Kaffir lime (Citrus hystrix) is a native plant of Indonesia. Nowadays the use of plants is limited as
ingredients in Indonesia cuisine and aromatherapy oils for industry. Leaves of C. hystrix contain of polyphenols
and essential oils thus they were possibly cytotoxic to cancer cell line. The objective of this research was to
determine the cytotoxicity effect and apoptosis induced by leaves extract C. hystrix to cervical cancer cell
line (HeLa cells). Methods used in this study included sampling of kaffir lime leaves, extractionusing three
different solvents (ethanol, ethyl acetate, and hexane), detection of metabolite secondary compound (alkaloids,
flavonoids, terpenoids, saponins, and tannins) using thin layer chromatography (TLC), cytotoxicity assay via
MTT assay, and apoptosis test with double staining method (ethidium bromide-acrydine orange). Result
showed kaffir lime crude extract dose dependently inhibitHeLa cells proliferation. lC50of ethanolicand ethyl
acetate extract was 82,034 ?g/mL and 57,845 ?g/mL, respectively means cytotoxic to HeLa cells. On the
other hand lC50 of hexane extract was 203,992 ?g/mL which was not cytotoxic. Furthermore ethanolic and
ethyl acetate extract were able to induce apoptosis of HeLa cells by increasing the number of apoptotic
cells. In conclusion, kaffir lime leaves extract had cytotoxic effect and induced apoptosis. Moreover ethyl
acetate extractof kaffir lime was the most potential to induce apoptosis in HeLa cells. |
