Karya
Judul/Title Cytotoxic activity of acidic Ribosome-inactivating Proteins Mirabilis jalapa L. protein (IP MJ-C) nanoparticle formulated with low chain chitosan and low methylated pectin
Penulis/Author Prof. Dr. apt. Sismindari, S.U. (2) ; Dr.rer.nat. Ronny Martien, M.Si. (3); Dr. Hilda Ismail, M.Si., Apt. (4); Dr. Agustinus Yuswanto, SU., Apt. (5)
Tanggal/Date 19 2017
Kata Kunci/Keyword
Abstrak/Abstract Mirabilis jalapa leaves were revealed to contain more than one ribosome inactivating proteins (RIP): RIP MJ-30 (basic RIP MJ) and MJ-C (acidic RIP MJ) [1-2]. Both of the RIPs have the ability to cleave supercoiled DNA into nick circular and linear form. It was found that RIP MJ-C has a more cytotoxic effect than RIP MJ-30 [3]. Therefore, RIP MJ-C could be developed as a potential anticancer agent. The challenge of RIP MJ-C delivery is that it could be easily degraded in the body and have potential difficulties in entering targeted cells [4-5]. Nanoparticle formulation would be one of the way to resolve these problems and improving protein activity against cancer cells [6-7]. Nanoparticles could be defined as colloidal particles with a size range of 10-1000 nm, forming a polymer wall or as a whole is formed as a matrix formed by polymers [8]. Nanoparticles formulated using biopolymers such as chitosan has been known to increase the effectivity of various anticancer drug delivery [9]. Previous work indicated that nanoparticle designed from low chain chitosan and high methylated pectin showed good potential for improving unpurified RIP MJ activity against MCF7 breast cancer cell-line [10]. A similar result was also found from unpurified RIP MJ nanoparticle formulated by medium chain chitosan and high methylated as well as low chained chitosan and alginate [11, 12]. Therefore, in this research, we explored the use of another formula using low chain chitosan and low methylated pectin as a delivery system for RIP MJ-C, a purified acidic RIP MJ protein. One of the ways to improve the selectivity and effectiveness of nanoparticle delivery system is by conjugating nanoparticles to molecules that could specifically reach target cancer cells. This was exemplified by conjugation of ribonucleic acid (RNA) A10 to poly (D,L-lactic-co-glycolic acid)-b-poly(ethylene glycol) (PLGA-b-PEG) nanoparticles containing cisplatin and dual-aptamer based doxorubicin delivery system for prostate cancer targeted therapy [13-15]. Other molecule target that might be used is epithelial cell adhesion molecule (EpCAM), a monomer membrane glycoprotein found within the normal human epithelium. EpCAM could serve as a molecular marker between cancer cells and normal cells since it was found to be over expressed on breast cancer cells [16]. By conjugating nanoparticle to an anti-EpCAM antibody, the toxic proteins or drugs would presumably be carried specifically into target cancer cells. Therefore, conjugation of anti-EpCAM antibody conjugation to RIP MJ-C nanoparticles could help carry RIP MJ-C to target cells without damaging the normal ones.
Rumpun Ilmu Farmasetika dan Teknologi Farmasi
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
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