Cytotoxic activity and apoptosis induction of 8-hydroxyisocapnolactone-2',3'-diol and its combination with doxorubicin on MCF-7 and T47D cells
Penulis/Author
Prof. Dr. apt. Ratna Asmah Susidarti, MS. (1); Dr. Riris Istighfari Jenie, M.Si., Apt. (2); Dr. apt. Muthi' Ikawati, M.Sc. (3); DYANINGTYAS DEWI PAMUNGKAS PUTRI (4); Prof. Dr. apt. Edy Meiyanto, M.Si. (5)
Tanggal/Date
2014
Kata Kunci/Keyword
Abstrak/Abstract
The cytotoxic activity and apoptosis induction of 8-hydroxyisocapnolactone-2 ?,3 ?-diol (HICD) and its combination with doxorubicin (Doxo) on MCF-7 and T47D cells have been evaluated. The cytotoxic assay was performed using MTT method. The IC50 was used to express the cytotoxic potency, while the combination index (CI) was calculated to determine the effect of combination. The apoptotic assay was carried out using acrydine orange - ethidium bromide double staining method, while Bcl-2 and Bax expression were investigated by immunocytochemistry. The HICD exhibited cytotoxic activity on MCF-7 and T47D cells with the IC50 of 8 ?g/ml (21.2 ?M) and 4 ?g/ml (10.6 ?M), respectively. The combination of HICD with Doxo showed synergistic effect and increased apoptosis induction on both cell lines. The HICD did not affect Bcl-2 but increased Bax expression on MCF-7 cells, while on T47D cells it suppressed Bcl-2 expression but did not modulate Bax expression
Rumpun Ilmu
Analisis Farmasi dan Kimia Medisinal
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Susidarti et al 2014.pdf
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2014-Susidarti et al.pdf
[PAK] Full Dokumen
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b_ JAPS Editorial board.pdf
[PAK] Halaman Editorial
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a_ JAPS-cover.pdf
[PAK] Halaman Cover
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f_ JAPS Scopus preview.pdf
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c_ JAPS 4(6) 2014-table of content.pdf
[PAK] Daftar Isi
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e_ Susidarti et al 2014-JAPS-similarity check Turnitin.pdf
[PAK] Cek Similarity
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Susidarti et al 2014-Scopus - Document details | Signed in.pdf
