| Penulis/Author |
Prof. Rumiyati, S.Si., Apt., M.Si., Ph.D. (1); Prof. Dr. Sismindari, S.U., Apt. (2); Prof. Dr. apt. Arief Nurrochmad, M.Si., M.Sc. (3); Prof. Dr. Endang Semiarti, M.S., M.Sc. (4); Prof. drh. Sitarina Widyarini, M.P., Ph.D. (5); DINAR PRASTIANDARI (6); ANDREA DHIETA UTAMA (7); DIANNI ANGGITA DEWI (8) |
| Abstrak/Abstract |
Abstract. Reactive oxygen species (ROS) exposure could lead to aging of the human cell. Tomato (Solanum lycopersicum L.) and carrot (Daucus carota L.) callus ethanolic extract are flavonoid-rich. Meanwhile tomato and carrot callus aqueous extract is suggested to contain protein that plays a role in cell regeneration against free radical. This study was aimed to evaluate the cytoprotective activity of tomato and carrot callus in preventing hydrogen peroxide
(H2O2)-induced cell cycle arrest in G0/G1 phase and inhibiting cell death. Cell death analysis of Human Dermal Fibroblast adult (HDFa) cells was performed using double staining method. Cell death inhibition showed in 0.15 mg · mL-1and 0.25 mg · mL-1 of tomato callus ethanolic extract (TCEE); 0.15 mg · mL-1 and 1.0 mg · mL-1 of tomato callus aqueous extract (TCAE); 0.15 mg · mL-1 and 0.25 mg · mL?1 of carrot callus ethanolic extract (CCEE); 0.05 mg · mL-1
and 0.50 mg · mL-1 of carrot callus aqueous extract (CCAE) pre-treatment compared with H2O2 control. The result of cell cycle analysis using FACS flows cytometry showed that 1.0 mg · mL-1 TCAE, 0.15 mg · mL-1 CCEE, and 0.5 mg · mL-1 pre-treatment inclines to reduce accumulation of cell in G0/G1. Keywords: carrot callus extract, cell cycle profile, cell death, human dermal fibroblast adult (HDFa) cell line, tomato, callus extract.
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