Cyclosporine A Decreases The Fluconazole Minimum Inhibitory Concentration of Candida albicans Clinical Isolates But Not Biofilm Formation and Cell Growth
Penulis/Author
Prof. dr. Tri Wibawa, Ph.D., Sp.MK(K) (1); dr. Nurrokhman, M.Si. (2); Baly, I. (3); Daeli, P.R. (4); Ginanjar Kartasasmita (5); Dr.biol.hom. Nastiti Wijayanti, S.Si., M.Si. (6)
Tanggal/Date
2015
Kata Kunci/Keyword
Abstrak/Abstract
Among the genus Candida, Candida albicans is the most abundant species inhumans. One of the virulent factors of C. albicans is its ability to develop biofilm. Biofilmforming microbes are characterized by decreasing of its susceptibility to antibiotics andantifungal. The fungicidal effect of fluconazole may be enhanced by cyclosporine A inlaboratory engineered C. albicans strains. The aim of this work is to analyze the synergisticeffect of cyclosporine A with fluconazole in C. albicans clinical isolates and the effect ofcycolsporine A alone in the biofilm formation. Six fluconazole resistant and six sensitive C.albicans clinical isolates were analyzed for its minimum inhibitory concentration (MICs),biofilm formation, and cell growths. A semi-quantitative XTT [2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide] reduction assay was conducted to measurethe biofilm formation. Cyclosporine A has synergistic effect with fluconazole that was shownby decreasing MICs of both fluconazole resistant and sensitive C. albicans clinical isolates.However, cyclosporine A alone did not influence the biofilm formation and cell growth of bothfluconazole resistant and sensitive C. albicans clinical isolates. These results indicated thatcyclosporine A might be a promising candidate of adjuvant therapy for fluconazole againstboth fluconazole resistant and sensitive C. albicans clinical isolates.
Rumpun Ilmu
Ilmu Kedokteran Tropis
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
176 - 182 Wibawa T.pdf
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Cyclosporine A decreases the fluconazole minimum inhibitory concentration of Candida albicans clinical isolates but not biofilm formation and cell growth.pdf