Karya
Judul/Title Curcumin and its Analogues (PGV-0 and PGV-1) EnhanceSensitivity of Resistant MCF-7 Cells to Doxorubicin throughInhibition of HER2 and NF-kB Activation
Penulis/Author Prof. Dr. apt. Edy Meiyanto, M.Si. (1) ; DYANINGTYAS DEWI PAMUNGKAS PUTRI (2); Prof. Dr. apt. Ratna Asmah Susidarti, MS. (3); drh. Retno Murwanti, M.P., Ph.D. (4); Prof. Dr. apt. Sardjiman, M.S. (5); ADITYA FITRIASARI (6); ULFATUL HUSNAA (7); Dr. Drs. Hari Purnomo, M.S., Apt. (8); Masashi Kawaichi (9)
Tanggal/Date 2014
Kata Kunci/Keyword
Abstrak/Abstract Chemoresistance of breast cancer to doxorubicin is mediated mainly through activation of NF-kB and overexpression of HER2. Curcumin and its analogues (PGV-0 and PGV-1) exert cytotoxic effects on T47D breastcancer cells. Suppression of NF-kB activation is suggested to contribute to this activity. The present studyaimed to explore the effects of curcumin, PGV-0, and PGV-1 singly and in combination with doxorubicin onMCF-7/Dox cells featuring over-expression of HER2. In MTT assays, curcumin, PGV-0, and PGV-1 showedcytotoxicity effects against MCF-7/Dox with IC50 values of 80 µM, 21 µM, and 82 µM respectively. Thesecompounds increased MCF-7/Dox sensitivity to doxorubicin. Cell cycle distribution analysis exhibited that thecombination of curcumin and its analogues with Dox increased sub G-1 cell populations. Curcumin and PGV-1but not PGV-0 decreased localization of p65 into the nucleus induced by Dox, indicating that activation of NF-kB was inhibited. Molecular docking of curcumin, PGV-0, and PGV-1 demonstrated high affinity to HER2 atATP binding site. This interaction were directly comparable with those of ATP and lapatinib. These findingssuggested that curcumin, PGV-0 and PGV-1 enhance the Dox cytotoxicity to MCF-7 cells through inhibition ofHER2 activity and NF-kB activation
Rumpun Ilmu Analisis Farmasi dan Kimia Medisinal
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1Edy-Meiyanto APJCP 2014.pdf
2Daftar isi_Asian Pacific Journal of Cancer Prevention - Articles List.pdf[PAK] Daftar Isi
3Meiyanto, 2014.pdf[PAK] Full Dokumen
4Curcumin and its Analogues (PGV-0 and PGV-1) Enhance Sensitivity of Resistant MCF-7 Cells to Doxorubicin through Inhibition of HER2 and NF-kB Activation.pdf[PAK] Cek Similarity
5Surat Keterangan Publikasi - Curcumin and its Analogues (PGV-0 and PGV-1) EnhanceSensitivity .pdfDokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)