| Abstrak/Abstract |
ABSTRACT: Diabetes mellitus often occurs in Indonesian society and people in various countries. Diabetes is known as a metabolic disorder characterized by an increase in sugar levels above normal. Metformin is an antidiabetic drug that has been declared safe and effective. Dayak onions (Eleutherine palmifolia (L.) Merr) are a typical Kalimantan plant that contains naphthoquinone derivative compounds that have anti-diabetic properties. This research aims to determine the relationship between the In-Silico Test and the In-Vivo Test for Naphthaquinone and Metformin Derivative Compounds and their Molecular Mechanism of Action as Antidiabetics. The research methods used were the PassOnline, STRING, and Molecular Docking approaches to determine the molecular mechanism and experimental testing was carried out on diabetic mice given Dayak onion extract and metformin to see changes in blood glucose levels. The results showed that the Napthaquinone derivative compound, namely 2-Anilino-1.4-Napthaquinone, when tested in silico, had a more stable docking score, namely -80.7631 compared to the native receptor ligand 1N1M -75.0595, and when 2-Anilino -1.4- Napthaquinone was compared with metformin produces a docking score of -82.6704 which is more stable than the metformin docking score of -66.7380 which works on the 3AQV protein. 2-Anilino-1.4-Napthaquinone can be a candidate for an antidiabetic drug that works on two receptors, namely 1N1M and 3AQV. The visualization results of 2-Anilino-1.4-Napthaquinone with the 3AQV receptor show many interactions bound to amino acid residues. In-vivo testing on single metformin, single Dayak onion, and a combination of both resulted in a reduction in blood glucose levels with a significance value of p <0.05 when compared with the negative control. |
