| Abstrak/Abstract |
Mosquito-borne diseases include tropical diseases such as malaria,
filariasis, dengue fever, chikungunya, yellow fever and cerebral fever are still
major health problems in Indonesia and on a global scale. Various methods
have been used to overcome this, including controlling vector mosquitoes using
the entomopathogenic microbial Lysinibacillus sphaericus. This study aims to
identify bacterial isolates collected based on the 16S rRNA gene and to carry
out the pathogenicity test of the bacterial isolates collected on Cx.
quinquefasciatus larvae. Bacterial isolates used in this study were collected
from root soil, bird droppings and guano. The identification of the type of
bacteria was carried out based on the 16S rRNA gene fragment. Based on the
results of the 16S RNA sequence analysis of isolates 229C, 6B4, 6.2 and 4D21,
it was found that the four isolates were included in the L. sphaericus species
with similarity scores ranging from 97% to 100%. The pathogenicity of
bacteria was measured based on the mortality of Cx. quinquefasciatus larvae to
know whether it has pathogenicity equal to or higher than strain 1593. The
pathogenicity test results of 6 isolates 15.4, 229C, 1593, 6B4, 6.2 and 4D21
showed that isolate 15.4 has the highest larval mortality rate, so it is potentially
used as a biological agent to control disease vector mosquitoes. |
