Karya
Judul/Title Conjugation of Anti-EpCAM Antibody on Alginate–RIP MJ-30 Nanoparticle through Carbodiimide Reaction as a Model of Targeted Protein Therapy
Penulis/Author Dr. Hilda Ismail, M.Si., Apt. (1) ; Prof. Dr. apt. Sismindari, S.U. (2); Dr.rer.nat. Ronny Martien, M.Si. (3); Dr. Agustinus Yuswanto, SU., Apt. (4)
Tanggal/Date 2 2019
Kata Kunci/Keyword
Abstrak/Abstract Ribosome inactivating proteins from Mirabilis jalapa L. (RIP MJ) has Revised: 4-1-2019 Accepted: xx-x-2019 *Corresponding author Hilda Ismail Email: hilda_fa@ugm.ac.id shown higher cytotoxic activity when being formulated as a nanoparticle. However, the selectivity of the delivery system is also an important aspect when it comes to cytotoxic cell therapy. Epithelial cell adhesion molecule (EpCAM) is a monomeric glycoprotein which is overexpressed in epithelial cancer cells. This study aim was to develop a model of targeted protein delivery system by formulating the base fraction of RIP MJ (RIP MJ-30) into alginate nanoparticles and conjugating it with anti-EpCAM antibody. RIP MJ- 30 was formulated in to nanoparticle using alginate and CaCl2 as cross- linker. Optimization of conjugation reaction condition was done in the pH variation of 4.5, 5.5, and 6.5. The success of conjugation was analyzed qualitatively using native polyacrylamide gel electrophoresis (native-PAGE) method and BCA assay. The optimum formula of RIP MJ-30 nanoparticles was produced using 0.3% alginate and 0.2?Cl2. Results indicated that optimum conjugation reaction was carried out at pH level of 5.5. The optimum native-PAGE condition was by using 8% polyacrylamide gel in duration of 6h. Characterization of nanoparticle resulted in particle size of 205.0nm, zeta potential of -6.9mV, entrapment efficiency of 71.11±4.84%, and conjugation efficiency of 89.55±6.18%. It was concluded that RIP MJ-30 was successfully formulated into alginate nanoparticle and conjugated to anti-EpCAM antibody through carbodiimide reaction using 1-ethyl- (dimethylprophilamine) carbodiimide (EDAC).
Rumpun Ilmu Farmasi Lain Yang Belum Tercantum
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1Paper Hilda-JIPharm_2019.pdf[PAK] Full Dokumen
21_Conjugation of Anti-EpCAM Antibody on Alginate–RIP MJ-30 Nanoparticle through Carbodiimide Reaction as a Model of Targeted Protein Therapy.pdf[PAK] Cek Similarity
3ilovepdf_merged (6).pdf[PAK] Bukti Korespondensi Penulis