Comparison on the Cancer Specific Cytotoxicity of Three Gingers (Zingiber officinale Rosc) Leaves Varieties from Indonesia
Penulis/Author
drh. Retno Murwanti, M.P., Ph.D. (2)
Tanggal/Date
2017
Kata Kunci/Keyword
Abstrak/Abstract
This study was performed to get more insight the cancer specific cytotoxicity of ginger leaf (GL). Three GL varieties (Gajah, Emprit, and Red) were extracted and fractionated. Each etyl acetate fraction in concentration of 200 μg/ml was tested about specific cytotoxicity toward cancer dan normal cells. Cancer cell lines used in this study were human colorectal (HCT116) and human breast (MCF-7 and T47D) cancer while normal cell line was human fibroblast (KMST-6). Based MTS assay method, the results showed Gajah and Emprit GL more significantly reduce cell viability of HCT116 and T47D than Red GL although there was no difference on the efficacy of both varieties. All varieties of GL also significantly reduce cell viability of MCF-7 compare to PBS control. However, there were not significant differences between those GL varieties on their effectiveness against MCF-7. In contrast, there were no effects on the KMST-6 due to all GL varieties treatment compare with PBS control. All data suggested that GL treatment only inhibited in the cancer cells without detrimental effect in the normal cells. Effectiveness of GL against cancer cell varies depend on the varieties. Gajah and Emprit GL are better varieties possess the cancer specific cytotoxicity that merits to be developed as promising chemo preventive agent in the future.
