| Abstrak/Abstract |
Paku Atai Merah tubers with the Latin name Angiopteris ferox Coupel have been used
by the Dayak people as empirical treatment and have anticancer and antioxidant activity. The
purpose of this study was to find out more the movement of extracts and fractions of Paku Atai
Merah tubers in reducing free radicals using the total antioxidant capacity (TAC), CUPRAC, and
Iron chelating methods. In this study, the sample used was the ethanol extract (EE) of A.ferox
Copel and then partitioned using n-hexane (FHP), ethyl acetate (FEAP), and aqueous-ethanol
(FEP) solvents. The effectiveness of the sample in reducing free radicals with various antioxidant
assay methods. The results obtained from this study indicate that the extract and fraction of Paku
Atai Merah tubers have good effectiveness as an antioxidant agent. The total antioxidant capacity
(TAC) method showed that EAP had a total antioxidant capacity of 101.60 µM/mg QEAC
compared to EE (44.62 µM/mg QEAC), FEAP (89.88 µM/mg QEAC), and FHP (81.21 µM/mg
QEAC). In the CUPRAC method using gallic acid as a standard for comparison, the results
obtained were EE (4.00 µM/mg GEAC), FHP (1.45 µM/mg GEAC), FEAP (8.66 µM/mg
GEAC), and APA (8.72 µM/mg GEAC). The antioxidant activity using the chelating iron
method in each sample of EE, HPA, EAP, and APA showed activity with IC50 values of 384
µg/mL, 387.41 µg/mL, 339.23 µg/mL, and 347.3 µg/mL. Based on the results obtained from this
study, it can conclude that the extract and fraction of Paku Atai Merah tubers have an excellent
antioxidant agent to develop as a food supplement.
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