| Abstrak/Abstract |
The purpose of this study was to compare the immune stimulating activity of dietary fucoidan from three Indonesian brown algae in white shrimp, Litopenaeus vannamei both at physiological and genetical levels. Six hematological parameters such as total hemocytes count (THC); phagocytic activity (PA); phagocytic index (PI); relative superoxide dismutase (SOD) activity; phenoloxidase (PO) activity; and total plasma protein (TPP) in Pacific white shrimp were evaluated after feeding using crude fucoidan from three genera of brown algae i.e. Sargassum, Padina, and Turbinaria that were collected from southern coastal waters in Gunung Kidul Regency, Indonesia at two different doses. The best fucoidan resource treatment was then re-tested to white shrimp to evaluate its effect on the expression of immune-related genes of shrimp including lipopolysaccharide-?-glucan binding protein (LGBP), Toll, lectin, and proPO by using qPCR. The results showed that dietary crude fucoidan from Sargassum was significantly able to increase THC, PA, and SOD activity in Pacific white shrimp. The treshold cycle (CT) analysis showed that dietary Sargassum crude fucoidan was also able to increase the expression of immune-related genes of shrimp, i.e. LGBP, Toll, and lectin. This study revealed that the crude fucoidan from tropical Sargassum was very promising to be developed as an immunostimulant in shrimp feed. |
