| Abstrak/Abstract |
Ionizing radiation (IR) increases risks to human health even in the low doses such as used in panoramic radiography by damaging DNA. Objectives: To assess the effect of X-ray radiation from panoramic radiography on DNA damage of buccal mucosa cells by comet assay. Methods: The total 20 participants were recruited from patients who received a panoramic examination in“Prof. Soedomo” Dental Hospital, Universitas Gadjah Mada. The subjects were divided into two groups comprised of 10 participants. The buccal mucosa cells were collected from all participants before exposure to IR, and 30 minutes or 24 hours after exposure in group 1 or group 2, respectively. DNA damage was then examined by comet assay. The comet assay output images were analyzed by OpenComet software. Results: The Double Strand Breaks (DSBs) were assessed by comparing tail % DNA presented in the OpenComet output image before and after X-ray exposure. The results pointed out a statistically significant (p= 0.014) increase in the percent of tail of DNA after 30 minutes of exposure, but not those exposed after 24 hours (p=0.29). Conclusion: Panoramic X-ray radiation may induce DSBs in 30 minutes after its exposure. |
