Colletotrichum scovillei Causing Chili Anthracnose in Yogyakarta, Indonesia and Its Pathogenicity
Penulis/Author
ROSA CHRYSE SUTOMO (1); Prof. Dr. Ir. Siti Subandiyah, M.Agr.Sc. (2); Dr. Ir. Arif Wibowo, M.Agr.Sc. (3); Prof. Ani Widiastuti, S.P., M.P., Ph.D. (4)
Tanggal/Date
30 2021
Kata Kunci/Keyword
Abstrak/Abstract
Chili anthracnose caused by Colletotrichum spp. was the significant disease of chili cultivation in Indonesia. Recent taxonomic status of Colletotrichum spp. has been rearranged due to well-developed of molecular study. Several species of Colletotrichum being re-identified after it was analyzed using polyphasic approach, inlcuding in Indonesia. The polyphasic approach which was a combination of morpho-cultural, molecular identification using ITS-rDNA and GADPH region, also pathogenicity test would be more reliable to identify of the species complexes of Colletotrichum, especially while morphology and molecular identifiation using ITS primer sets could not clearly differentiate of the C. acutatum complex species. The aims of this study were to identify the Colletotrichum species causing chili anthracnose, especially in fruits collected from chili field in Yogyakarta, Indonesia and observe its pathogenicity in wounded and unwounded method of inoculation. Based on the combination of methods shown that isolates were collected from chili fields in Yogyakarta had high similarity to C. scovillei. The pathogenicity test also revealed that the fungus caused anthracnose disease in both wounded and unwounded of OR Twist 42 chili fruits. The unwounded infection was confirmed by amplification of pathogenicity gene CheC3 on 24 h after inoculation showed that appressoria production may possess important role in the unwounded inoculation. This study revealed that C. scovillei does not always need a wound for its pathogenicity.
