| Abstrak/Abstract |
Objective(s): To investigate the effect of cocoa on orthodontic tooth movement (OTM) rate,
osteoprotegerin (OPG), and receptor activator of nuclear factor ? ? ligand (RANKL) levels after
OTM.
Materials and Methods: A total of 24 Sprague Dawley rats were included in the study. They
were equally divided into two groups: cocoa and control. The upper incisors of all rats have
subjected to orthodontic force 35 cN and moved distally with a stainless steel 3-spin coil spring.
During OTM, the cocoa group was given 4.8 grams of unsweetened cocoa once a day. At 4
subsequent time points (0, 1, 7, and 14 days), the OTM rate was determined by measuring the
distance between the mesial tips using a digital caliper, while OPG and RANKL levels were
examined based on their gingival crevicular fluid through specific enzyme-linked
immunosorbent assay (ELISA). Data gathered were analyzed using an independent t-test (P <
0.05).
Results: The OTM rate of the cocoa group was significantly higher than that of the control group
on days 1, 7, and 14 (P < .05). ELISA analysis revealed that the OPG level was significantly
lower on day 14. Furthermore, the RANKL level was significantly higher on day 0, 1, and 7 for
the cocoa group compared with the control group (P < .05).
Conclusion: These results indicate that cocoa has the potential effect to modulate the OTM rate
by inducing osteoclastogenesis, which suppresses the OPG level and stimulates the RANKL
level, in rats. |
