| Abstrak/Abstract |
Milk is a highly nutritious food containing several essential nutrients, consequently, it can be contaminated by several pathogenic bacteria, including Staphylococcus aureus (S. aureus). This study aimed to analyze the genes encoding the nine classical enterotoxins of S. aureus isolates derived from cows and goats raw milk. A total of 75 S. aureus isolates were obtained from milk samples of dairy cows (46 isolates) and Peranakan Etawa (PE) goats (29 isolates) from different farms in Yogyakarta Indonesia. Based on the cultural and biochemical properties and the amplification of the 23S rRNA specific to S. aureus, all isolates could be identified as S. aureus. Amplification of the staphylococcal enterotoxin genes was used multiplex polymerase chain reaction (PCR). The seh gene was the most frequently observed in 21 isolates (45.65%) of cows, followed by sea gene (8 isolates, 17.39%), seg and sei genes (4 isolates, 8.69%). Among the S. aureus isolated from goats, there was only sec gene observed in 2 isolates (6.89%). The distribution of staphylococcal enterotoxin genes from ready to eat food (dairy product) is of our concern. |
