| Abstrak/Abstract |
Chlorogenic acid (CGA) is known to have antioxidant potentials, yet the effect of
CGA on brain ischemia has not been sufficiently understood. Brain ischemia such as
transient global ischemia disrupts many areas of the brain of rats, including the hippocampus.
Male Wistar rats were randomly assigned into five groups, that is, shamoperated
(SO), bilateral common carotid occlusion (BCCO), and BCCO+ 15, 30,
and 60 mg/kg bw CGA groups (CGA15, CGA30, and CGA60, respectively). Brain
ischemia was induced in Wistar rats with BCCO for 20 min followed by intraperitoneal
injection of CGA. The rats were examined for the spatial memory in a Morris
water maze test on the 3rd day and were euthanized on the 10th day after BCCO. The
total number of pyramidal cells was estimated, and the mRNA expressions of Bcl2,
Bax, caspase?3, SOD2, SOD1, GPx, ET?1, eNOS, CD31, and VEGF?A were measured.
The BCCO group spent less time and distance in the target quadrant than any
other group in the spatial memory retention test. The CA1 pyramidal cell numbers in
the BCCO and CGA15 groups were lower than in the CGA30 and CGA60 groups.
The mRNA expressions of Bcl2, SOD2, and CD31 in the BCCO group were lower
than in the CGA15, CGA30, and CGA60 groups. The ET?1 expression was higher
in the BCCO and CGA15 groups than in the SO, CGA30, and CGA60 groups. CGA
improves the spatial memory and prevents the CA1 pyramidal cell death after BCCO
by increasing Bcl2, SOD2, and CD31 expressions and decreasing ET?1 expression. |
