Karya
Judul/Title Chitinase activity of Pseudomonas stutzeri PT5 in different fermentation condition
Penulis/Author NIHLAH CHALIDAH (1); IZFARANI NUR K (2); ARIF ROHMAN KHAKIM (3); BHATARA AYI MEATA (4); Indun Dewi Puspita, S.P., M.Sc. Ph.D. (5) ; Dr. Prihati Sih Nugraheni, S.Pi., M.P. (6); Prof. Dr. Ir. Ustadi, M.P. (7); Sri Pudjiraharti (8)
Tanggal/Date 2018
Kata Kunci/Keyword
Abstrak/Abstract This study aimed to determine the incubation condition of Pseudomonas sp. PT5 in producing chitin degrading enzyme in various pH and temperatures; to compare the production of chitin degrading enzyme in chitin medium supplemented with additional nitrogen, carbon, and mixture of nitrogen and carbon sources; and to compare the incubation condition to produce chitin degrading enzyme in 250 ml-shaken flask and 2 L- fermentor. The parameters tested during production were chitinase activity (U/mL) and the concentration of N-Acetylglucosamine (NAG) in the media (mg/mL) measured by a spectrophotometer colorimetric method, using wavelength of 584 nm. The results showed that Pseudomonas PT 5 was able to produce highest chitinase activity at pH 6 and temperature of 37oC (0.024 U/ml). Pseudomonas sp. PT5 was able to utilize nitrogen sources of 0.05 % ammonium phosphate and carbon sources of 0.1 % maltose to produce the highest chitinase activity at day-4 incubation of 0.030 U/ml and 0.029 U/ml, respectively. Compare to the solely supplemented of whether additional nitrogen or carbon sources to chitin medium, the additional of 0.1 % ammonium phosphate and 0.1% maltose mixture to chitin medium resulted to the shorter time of chitinase production to day-3 of incubation at 250 ml-shaken flasks (0.021 U/ml). The production of chitinase using 2 L-fermentor shows that the highest chitinase activity produce by Pseudomonas PT5 was reached at 1-day incubation (0.0283 U/ml), which was shorter than in 250 ml-shaken flasks. The study to optimize the ration of ammonium phosphate and maltose mixture is required to reach higher chitinase production by Pseudomonas sp. PT5.
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