Characterization of Kacang Goat skin Pepsin Soluble Collagen (Psc) and Their Potency as an Antioxidant
Penulis/Author
Dr. Rina Wahyuningsih (1); Ir. Rusman, M.P., Ph.D. (2); Prof. Dr. Ir. Nurliyani, M.S., IPM (3); Prof. Dr. Abdul Rohman, S.F., M.Si., Apt. (4); Prof. Ir. Nanung Agus Fitriyanto, S.Pt., M.Sc., Ph.D., IPM (5); Prof. Ir. Yuny Erwanto, S.Pt., M.P., Ph.D., IPM (6)
Tanggal/Date
2021
Kata Kunci/Keyword
Abstrak/Abstract
Collagen have been interesting material for many utilization such as food, pharmaceutical
and cosmetic in various products and target administration, consequently collagen should be
prepared as well as type of application. The objective of this research is to prepare collagen
from goat skin and investigate the character and their potency as an antioxidant. Kacang goat
skin aged 2 years was used for collagen production. Small slice skin was extracted by curing
with 0.1% (w/v) pepsin in acetic acid 0.5 M, for 24, 48, dan 72 h at 4°C. The variables observed
were molecular weight by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS
PAGE), microstructure using scanning electron microscope, thermal stability by differential
scanning calorimetric, and the antioxidant potency through 2,2-diphenyl-1-picrylhydrazyl
(DPPH) inhibition analysis. The result showed the molecular weight range from 25 kDa to 180
kDa, microstructure showed the collagen fibril crosslink, collagen start to denature at 62,28°C,
highest dissolved with 1% NaCl concentration and has highest DPPH inhibition at 60 min after
hydrolysis. In conclusion, pepsin soluble collagen derived from kacang goat skin has
antioxidant capacity and could be used in some applications.
Rumpun Ilmu
Teknologi Hasil Ternak
Bahasa Asli/Original Language
English
Level
Nasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
[PAK] Characterization of kacang Goat Skin Full Document.pdf
[PAK] Full Dokumen
2
[PAK] CHARACTERIZATION OF KACANG GOAT SKIN Similarity.pdf
[PAK] Cek Similarity
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sertifikat-jitek.pdf
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