Characterization of envelope-transmembrane Gene of Jembrana Disease Virus Tabanan 1995 Isolate.
Penulis/Author
Prof. Dr. drh. Asmarani Kusumawati, M.P. (1); Prof. Dr. Rarastoeti Pratiwi, M.Sc. (2); Dr. drh. Pudji Astuti M.P. (3); Dr. drh. Penny Humaidah Hamid, M. Biotech (4)
Tanggal/Date
2010
Kata Kunci/Keyword
Abstrak/Abstract
The availability of specifi c and rapid detection methods is essential for monitoring the health statusof farmed species, particularly in viral disease as in this case early diagnosis is a critical factor in containing disease outbreaks. Jembrana Disease Virus (JDV) is a lentivirus that causes an acute, severe disease syndrome in infected Bali cattle in Indonesia, resulting in heavy economic losses because of the high mortalities. The virus-host interaction and the modes of transmission are still unknown. The goal of the research was to design a probe candidate of Jembrana Disease Virus based on envelope-transmembrane (env-tm) gene to optimize Jembrana disease detection method. The DNA fragment derived from env-tm of JDV was used, cloned in pGEX-TM and expressed in E.coli DH 5?. Sequence analysis was conducted with BLAST programs from NCBI. Sequence analyses of the fragments of env-tm clone, indicated that it has a very closed genetic relation with 97,68% homology identity. Probe was designed based on the conserved region of env-tm using Geneious resulted in JT2 252 bp long. BLAST analyses showed that probes had high specifi ty to other strains of JDV in Indonesia
Rumpun Ilmu
Biologi (dan Bioteknologi Umum)
Bahasa Asli/Original Language
English
Level
Nasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Characterization of envelope-transmembrane Gene of Jembrana Disease Virus Tabanan 1995 Isolate.pdf
[PAK] Full Dokumen
2
SK 2010.pdf
Dokumen Pendukung Karya Ilmiah (Hibah, Publikasi, Penelitian, Pengabdian)
