Characterization of buccal cell DNA after exposure to azo compounds: a cross-sectional study
Penulis/Author
Prof. Dr. drg. Juni Handajani, M.Kes., Ph.D (1); Urfa Tabtila (2); NADIA RULLY A (3); Prof. Dr. Abdul Rohman, S.F., M.Si., Apt. (4)
Tanggal/Date
2020
Kata Kunci/Keyword
Abstrak/Abstract
Background: Azo compounds, containing naphthol and diazonium
salts, are synthetic dyes widely used in the batik industry. Azo
compounds are considered toxic when they are exposed to human
tissue. The purpose of this study was to analyze buccal cell DNA
exposed to azo compounds in batik workers.
Methods: A cross-sectional study involving 20 male subjects divided
into two groups (n=10 group), namely azo-exposed and non-exposed
(control group). Inclusion criteria were batik workers of the colouring
division who have been exposed to azo for at least 5 years. Buccal
cells were taken using cytobrush then DNA were isolated from buccal
cell. DNA isolation was done by buccal DNA kit, while the purity and
concentration of the DNA was determined using spectrophotometer
and electrophoresis.
Results: The azo-exposed group revealed higher purity DNA than
those in the control group. The purity of the DNA in the azo-exposed
group and control group was 0.61±0.93 and 0.21±0.09, respectively,
while the concentration of DNA was of 59.02 and 19.35 ng/UL,
respectively. The ratio at 260/280 nm was 1.84-1.94 (azo-exposed) and
1.85-1.92 (control). Principal component analysis using the first
principle component (PC1) and second principle component (PC2)
could successfully classify subjects in the control and azo-exposed
groups.
Conclusion: Characteristics of DNA could be used as an indication of
exposure to azo compounds in workers of batik industries.
