| Abstrak/Abstract |
This study aimed to characterize and assess the immunomodulatory capability of exopolysaccharides (EPSs) isolated
from Lacticaseibacillus casei AP and AG. In a batch fermentation model, L. casei AP produced 48 mg/L EPSs, whereas L.
casei AG produced 62 mg/L EPSs. The percentages of total carbohydrates in EPSs from L. casei AP and L. casei AG were
79.40 and 81.17%, respectively. Monosaccharide composition analysis revealed the presence of ribose and glucose in EPSs
produced by both strains. Fourier transform infrared (FTIR) spectroscopy confirmed the presence of hydroxyl, carboxyl, and
amide groups. EPS microstructures showed coarse surfaces, with the main constituents being C, N, O, and Ca. In addition, P
was present in the EPSs produced by L. casei AP but absent in the EPSs produced by L. casei AG. After grown on standard
MRS medium, the EPSs isolated from L. casei AP and L. casei AG exhibited different immunomodulatory activities. These
EPSs effectively increased the phagocytic and nitride oxide production of RAW 264.7 cells without toxic effects at concen‑
trations between 50 and 800 μg/mL, suggesting that these strains are interesting producers of EPSs, which could be used in
various potential applications in the food and pharmaceutical industries. |
