Karya
Judul/Title CCL2 and IL18 expressions may associate with the anti-proliferative effect of noncontact electro capacitive cancer therapy in vivo
Penulis/Author Prof. Dr. Rarastoeti Pratiwi, M.Sc. (1) ; NYOMAN YUDI ANTARA (2); Lalu Gunawan Fadliansyah (3); Syamsul Arif Ardiansyah (4); Luthfi Nurhidayat, S.Si., M.Sc. (5); dr. Eti Nurwening Sholikhah M.Kes. (6); Prof. Dr. Dra. Sunarti, M.Kes. (7); drh. Sitarina Widyarini, M.P., Ph.D. (8); Ahmad Ghitha Fadhlurrahman (9); HINDANA FATMASARI (10); Woro Anindito Sri Tunjung, S.Si., M.Sc., Ph.D. (11); Prof. dr. Sofia Mubarika Harjana, M.Med.Sc., Ph.D. (12); Firman Alamsyah (13); Warsito Purwo Taruno (14)
Tanggal/Date 17 2019
Kata Kunci/Keyword
Abstrak/Abstract Background: Noncontact Electro Capacitive Cancer Therapy (ECCT) is a novel treatment modality in cancer. Chemokine (C-C motif) ligand 2 (CCL2) has a major role in the outgrowth of metastatic breast cancer. Interleukin 18 (IL18) plays a role in macrophage alteration, which leads to excessive angiogenesis. This study aims to elaborate on the association of CCL2, IL18, IL23?, and TNF-? (tumor necrosis factor-alpha) expression with the anti-proliferative effect of ECCT in rat breast tumor tissue. Methods: Low intensity (18 Vpp) and intermediate frequency (150 kHz) alternating current-electric field (AC-EF) between two capacitive electrodes were exposed as external EF to a rat cage. Twenty-four rats were divided into four groups of six replicates. Breast tumor tissues were collected from 7, 12-dimethylbenz[a]anthracene (DMBA)-induced rats. Two groups were none DMBA-induced rats without ECCT exposure (NINT) and with (NIT). The other two groups were DMBA-induced rats without ECCT exposure (INT) and with (IT). Mammary glands and breast tumor tissues were collected from each group and preserved. Hematoxylin-eosin and immunohistochemistry staining were performed on paraffin sections of tissues using anti-PCNA, anti-ErbB2, anti-Caspase3, and anti-CD68. CCL2, IL18, IL23?, and TNF-? mRNA relative expressions were analyzed using qRT-PCR. Results: ECCT exposure may cause the reduction of PCNA protein expression as well as ErbB2 on breast tumor tissues, but it causes the increase of Caspase3 and macrophage CD68 protein. In rat breast tumor tissues of IT groups, the mRNA expression of CCL2 and IL18 are significantly down-regulated, in contrast with the up-regulated expression of these cytokines in tumor tissues of the INT group. IL23? and TNF- ? expression remained similar in both groups. Conclusion: CCL2 and IL18 expressions have an association with the inhibition of breast tumor cell proliferation affected by ECCT exposure
Rumpun Ilmu Ilmu Kedokteran Dasar & Biomedis
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
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