| Abstrak/Abstract |
Diabetes mellitus (DM) is a metabolic disorder with symptoms of chronic hyperglycemia and disturbances in metabolism of carbohydrates, fats and proteins due to insulin deficiency, insulin resistance or inability of ?-cells of pancreas to produce insulin. This study was aimed to evaluate the effects of ethanolic extract from Sargassum polycystum on glucose level and lipid profile in streptozotocin-induced diabetic rats. S. polycystum was extracted with ethanol 96%, then the extract was tested for in vivo experiment and analyzed using GC-MS. Ethanolic extract of S. polycystum at 150 mg/kg and 450 mg/kg was administered orally to streptozotocin induced diabetic rats, once daily for 15 days. The results obtained were then compared with normal controls (non-diabetes+normal saline 0.9% p.o), negative control (diabetes +CMC-Na 0.5% p.o) and positive control (diabetes+glibenclamide 5 mg/kg p.o). The glucose levels, lipis profile and body weight were measured on normal condition (baseline), on 0, 5th, 10th, 15th day, and pancreatic histopathological study was done on 15th day. Result showed that S. polycystum extract with 150 mg/kg had the highest capability on prevention of DM indicated by the increase in body weight, decreased of blood glucose values, increased of cholesterol values, decreased of triglyceride values, increased of HDL-c and LDL-c. Pancreatic necrosis was found in all streptozotocin-induced rats. S. polycystum extract with 450 mg/kg concentrations showed highest reduction of ?-cells of the pancreas induced by streptozotocin is better than S. polycystum extract with 150 mg/kg. S. polycystum extract contains terpenoids and phenolic such as camphor, elemol, ?-elemene, ?-curcumene, germacrone and phenol which might be potential as oral antidiabetes therapy. |
