| Abstrak/Abstract |
The urgency of multidrug-resistant (MDR) bacterial infections in wounds is asignificant concern due to the high prevalence of MDR in healthcare settings.Black pigmented marine bacteria, strain PS1C, were isolated from marinesediment on Awur Beach Jepara, Central Java, Indonesia, and cultured in thelaboratory. The aim of this research includes molecular identification of strainPS1C, extracting black pigment from strain PS1C, isolation of MDR bacteriafrom wounds, and evaluating the antibacterial activity of black pigment fromstrain PS1C against MDR bacteria isolates of wound infections. We examinedthe 16S rRNA gene sequences strain PS1C to identify the species. Then, theblack pigment from PS1C was extracted using methanol: acetone (7:3) solvent.Antibacterial activity was evaluated against MDR bacteria from wounds withthe microdilution method. A black-pigmented bacterium was isolated andidentified asPseudomonas aeruginosaPS1C. We found that the black pigmentfromP. aeruginosaPS1C can be developed as an antibacterial agent against theMDR bacterial isolate of wounds with MIC and MBC values of 6.25-12.5 mg/ml and 6.25-25 mg/ml, respectively. In conclusion, the study's findings highlightthe potential of the extract of black pigment fromP. aeruginosaPS1C as anantibacterial agent against wound-causing MDR bacteria and reinforce previousresearch intoP. aeruginosacan be isolated from marine sources. Additionalinvivoinvestigations and the identification of the antibacterial activity's mode ofaction are required.
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