| Abstrak/Abstract |
The drug-herb combination between metformin and bitter melon frequently showed potential effects such as lowering glucose level. This study aims to analyze metformin in plasma sample using HPLC method and its pharmacokinetic profiles affected by bitter gourd. The study was conducted using an experimental method with post-test randomized controlled group design. Healthy albino rabbits were divided into three groups and were administered with bitter gourd juice 100% (v/v) and metformin (BM1), bitter gourd juice 50% (v/v) and metformin (BM2), and metformin 26 mg/kg (M). BM1 and BM2 was given bitter gourd juice for 14 days, then a single dose of metformin was given to all groups on the fifteenth day before metformin pharmacokinetic parameters were measured. The plasma was analyzed using HPLC methods then the concentration versus time used for pharmacokinetic analysis. Bitter gourd fruit juice with 100% (v/v) concentration affected the pharmacokinetic profile of the metformin, decreased the V/F and CL/F, also increased Ka, T1/2, Cmax, MRT, and AUC0-inf, also significantly increased AUC0-480, and decreased Tmax (p < 0.05). Pharmacokinetics parameters of metformin after extravascular input could be analyzed with 1-compartmental open model analysis. Bitter gourd fruit juice with 100% (v/v) concentration affected the pharmacokinetic profile of the metformin. It was significantly increased AUC0-480, and decreased the Cmax of metformin. Based on the result, this can be used as consideration to combine metformin and bitter gourd for diabetes treatment and metformin dose adjustment also resulted in beneficial pharmacokinetics interaction in normal condition. |
