Bioactivity of Fungi Trichoderma reesei Associated with Sponges Stylissa flabelliformis Collected from National Park West Bali, Indonesia
Penulis/Author
Prof. Dr. apt. Erna Prawita Setyowati, M.Si. (1); Dr. Sylvia Utami Tunjung Pratiwi, S.Si., M.Si. (2); Prof. Dr.rer.nat. apt. Triana Hertiani, S.Si., M.Si. (3); Oka Samara (4)
Tanggal/Date
2017
Kata Kunci/Keyword
Abstrak/Abstract
Objective: In this study, antimicrobial and cytotoxic properties of ethyl acetate extract of fermented Trichoderma reesei(T. reesei)fungi
associated with the sponge Stylissa flabelliformis (S. flabelliformis) was tested. Materials and Methods: Fermentation of T. reeseiwas
carried out in the saboroud dextrosa saline broth. The fermented filtrate was extracted using ethyl acetate. The ethyl acetateextract was
tested for its antimicrobial and cytotoxic activity. Thin-layerchromatography and gas chromatography mass spectrometry were performed
to determine the metabolite classes of compounds responsible for antimicrobial and cytotoxic activity. Results: Ethyl acetate extract
of T. reesei was active against the bacteria Staphylococcus aureusATCC 29213, Eschericia coli ATCC 25922 and Candida albicansATCC
10231. The ethyl acetate extract was also active against cancer cells T47D and Raji cells, with IC50 values of 270 and 470 µg mLG
1
,
respectively. Conclusion: An ethyl acetate extract of Trichoderma reeseif er ment at ions showed ant imicr obial and cyt ot oxic act ivit y. The
compounds contained in the extract of T. reesei are terpenoids or phenyl propane derivatives.
Rumpun Ilmu
Biologi Farmasi
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Bioactivity of Fungi Trichoderma reesei Associated with Sponges Stylissa flabelliformis Collected from National Park West Bali, Indonesia.pdf
2
Bioactivity of Fungi Trichoderma reesei Associated with Sponges Stylissa flabelliformis Collected from National Park West Bali, Indonesia.pdf