Karya
Judul/Title Bioactivity and genetic screening of marine actinobacteria associated with red algae Gelidiella acerosa
Penulis/Author MARIA ULFAH (1); Apt Noer Kasanah, S.Si., M.Si., Ph.D. (2) ; Prof. Dr. Niken Satuti Nur Handayani, M.Sc. (3)
Tanggal/Date 2017
Kata Kunci/Keyword
Abstrak/Abstract Bacterial resistance to existing antibiotics has driven a search for new antibiotics from marine actinobacteria. Bioactivity and genetic screening of actinobacteria associated with red algae Gelidiella acerosa were conducted to discover new antibacterial compounds against Vibrio alginolyticus. A total of 14 actinobacteria isolates were obtained from G. acerosa. The isolates were subjected to genetic screening for nrps (non-ribosomal peptide synthetase) and FADH2-dependent halogenase genes. The isolates’ ability to produce secondary metabolites was examined by fermentation in various media in a six-well mini plate. The bioactivity of the secondary metabolites was screened using a microtiter assay and the agar overlay method. The results showed that all 14 isolates had the nrps gene, whereas none had the halogenase gene. Meanwhile, eight of the actinobacteria isolates showed antibacterial activity against V. alginolyticus. Keywords actinobacteria, antibiotic, FADH2-dependent halogenase, NRPS, red algae
Rumpun Ilmu Bioteknologi Perikanan
Bahasa Asli/Original Language English
Level Nasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
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