| Penulis/Author |
Prof. Dr. Tri Joko, S.P., M.Sc. (1) ; Dewi Nur Anggraeni (2); Mery Irianti (3); Prof. Dr. Budi S. Daryono, M.Agr.Sc. (4); Ir. Jaka Widada, M.P., Ph.D. (5); Prof. Dr. Ir. Siti Subandiyah, M.Agr.Sc. (6) |
| Abstrak/Abstract |
The intimate relationship between endophytic bacteria and their host may influence the physiological mechanisms of plants. In this study, a hundred and six bacterial endophytes were isolated from tissues of twelve orchid species, and screened according to in vitro antagonistic activity, indole-3-acetic acid (IAA) production, and phosphate solubilization. Among 106 isolates able to inhibit soft-rot pathogen, 79 were found to produce IAA and 92 were able to solubilize inorganic phosphate. Thirty selected isolates were then subjected to ERIC and rep-PCR to investigate their genetic diversity. Three promising isolates (AkOc1, DnAr4, and TbPh7) were then applied for in vitro growth and challenged against soft rot bacterial pathogen. It was found that introduction of endophytic isolates to plantlets of Phalaenopsis amabilis increased significant growth of plantlet as well as disease suppression. Characterization by partial 16S rRNA gene sequences analysis revealed that the isolates were closely related to Raoultella ornithinolytica, Klebsiella michiganensis, and Bacillus amyloliquefaciens subsp. plantarum respectively. The ability of the isolates to inhibit soft rot bacterial pathogen as well as to promote plant growth makes them potential for enhancing plant health. |
