| Penulis/Author |
Prof. Ir. Donny Widianto, Ph.D. (1) ; AJENG DARA PRAMITA (2); IRIANTI KURNIASARI (3); Nur Akbar Arofatullah, S.P., M.Biotech., Ph.D. (4); Prof. Ir. Irfan Dwidya Prijambada, M.Eng., Ph.D. (5); Ir. Jaka Widada, M.P., Ph.D. (6); Prof. Dr. Ir. Siwi Indarti, M.P. (7) |
| Abstrak/Abstract |
The nematicidal ability of forty-five bacterial isolates from potato cultivation lands in Indonesia were examined. The isolates showing >=50% cyst and egg mortality were identified based on morphological and molecular approach. DK-Sa-A1, SRJ-Sa-A1, KB-Se-A2, and SRJ-Se-A1 showed cyst mortality of 80, 63.3, 53.3, and 50%, respectively. The ovicidal potential of SRJ-Se-A1, DK-Sa-A1, SRJ-Sa-A1, KB-Se-A2,KB-Sa-B3, KT-Se-A2, and PB-Sa-A2 were 98.7, 96.4, 95.2, 94.1, 76.4, 70.3, and 54.4% egg mortality, respectively. Five isolates representing each sampling location, DK-Sa-A1, KB-Se-A2, KT-Se-A2, PB-Sa-A2, and SRJ-Sa-A1 were selected for further analysis. These isolates showed significantly different protease and chitinase specific activity compared with the control and were identified as Bacillus flexus DK-Sa-A1, B. pumilus KB-Se-A2, B. cereus KT-Se-A2, B. megaterium PB-Sa-A2, and B. subtilis SRJ-Sa-A1. All identified isolates were not plant pathogenic and did not trigger a hypersensitive response. Therefore, they could be potential biocontrol agents for golden cyst nematode. |
