Application of silica extracted from rice husk ash for the encapsulation of AFB1 antibody as a matrix in immunoaffinity columns
Penulis/Author
Dr. Deni Pranowo, S.Si., M.Si. (1); Prof. Dr.rer.nat. Nuryono, M.S. (2); Prof. Dr. Ir. Ali Agus, DAA., DEA., IPU., ASEAN Eng. (3); Prof. Drs. Jumina, Ph.D. (4); Romsyah Maryam (5); Dr.nat.techn. Francis M Constance Sigit Setyabudi, S.T.P., M.P. (6)
Tanggal/Date
2017
Kata Kunci/Keyword
Abstrak/Abstract
The extraction of silica from rice husk ash (RHA) for the encapsulation
of aflatoxin B1 antibody (Ab-AFB1) and its application as a matrix in immunoaffinity
columns (IACs) were achieved. The RHA extraction was performed
using 4 M NaOH, which yielded sodium silicate (Na2SiO3) for the synthesis
of silica gel. The obtained silica was used for encapsulating Ab-AFB1 using
the sol-gel technique. One milliliter of 1 M Na2SiO3:H2O:H3PO4 (0.43:0.11:
0.46) could generate silica gel that was suitable for encapsulating 1.36 mg of
Ab-AFB1 at pH 7. After 48 hours of aging, the silica gel modified with AbAFB1
(SG-Ab-AFB1) was ground, and packed as the matrix in the IAC for aflatoxins
purification. The modified silica gel was characterized using FTIR and
SEM. The properties of IAC with SG-Ab-AFB1 were investigated by evaluating
AF recovery, binding capacity, and reusability. The recovery of AFB1 was
94.11 ± 4.62%. In addition to AFB1 recovery, the column also retained AFB2,
AFG1, and AFG2 with recovery values of 98.22 ± 3.74%, 92.22 ± 7.62%, and
83.00 ± 6.31%, respectively. This column, which contained 0.5 g of SG-AbAFB1
had a binding capacity of approximately 50 ng of AFs per column, and
could be reused at least 5 times with a recovery of more than 80%.
Rumpun Ilmu
Kimia
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
J11_9_2017_Application of silica extracted from rice husk ash for_Full Dokumen_1.pdf
[PAK] Full Dokumen
2
J11_5_2017_Application of silica extracted from rice husk ash for_JSM_Similarity.pdf
