| Abstrak/Abstract |
Green tea (Camellia sinensis L.) contains bioactive compounds such as epigallocatechin gallate (EGCG), ca?eine, and
gallic acid. The study aimed to optimize the extraction condition using the experimental design of factorial design.
Two variables namely water temperature (75 and 95oC) and brewing number (one and two-times) were used and
objected to factorial design in order to get the optimum condition. The determination of EGCC, ca?eine, and gallic
acid was carried out using high-performance liquid chromatography method equipped with the UV-visible detector.
The result showed that the extraction yield varied from 4.48%-7.56%. The level of EGCG and ca?eine in green tea
extract varied from 251.96-393.34 mg/g dry weight and 32.94-46.82 mg/g dry weight, while the level of gallic acid
could not be quantifed in each experiment because it was below the limit of quantifcation (LOQ). The predicted
optimum extraction condition consisted of water temperature at 95oC with two-times brewing. Using this optimum
condition, the concentrations of EGCG, ca?eine and the extraction yield were of 356.43 mg/g dry weight, 38.76 mg/g
dry weight, and 5.76%, respectively. |
