Judul/Title
Antiplasmodial Activity of Endophytic Fungi isolated fro Artemisia annua L.
Penulis/Author
Dr. apt. Indah Purwantini, S.Si., M.Si. (1) (2) (3) (4)
Tanggal/Date
2016
Kata Kunci/Keyword
Abstrak/Abstract
his research will observed the kinetics of fungi’s growth and the ability of endophytic fungi IP-2 and IP-6 to inhibit the growth of Plasmodium falciparum strain FCR-3. Methods: IP-2 and IP-6 were fermented in Potato Dextrose Broth (PDB) medium for 14 days at room temperature and sampling was done every 24 hours to obtain biomass. Sspecific growth rate (?) was estimated from biomass during logarithmic phase and compared for different microorganisms. The extract of medium fermentation was obtained by extracted using ethyl acetate and then evaporated untill sticky in its consistency. The extracts were subjected to antiplasmodial activity using continuous culture of P. falciparum method which developed by Trager and Jensen. Upon reaching the desired parasitaemia, the culture was synchronized refer to Lambros and Vanderberg method. Antiplasmodial assay was performed for 72 hours. At the end of incubation, parasitaemia was determined using Giemsa-stained thin blood smears and observed using microscope. The potency of antiplasmodial activity was expressed as IC50 which calculated by probit analysis. Results: Result showed that specific growth rate for IP-2 and IP-6 were 0.04 h-1 and 0.01 h-1. Both endophytic fungi had activity as an antiplasmodial with IC50 values 42.58±5.65 and 30.08±4.73 ?g/mL respectively. Conclusion: IP-2 showed faster growth rate than IP-6. At 168 hours of fermentation, growth rate of IP-2 entered stationary phase while IP-6 216 hours. Endophytic fungi IP-2 and IP-6 demonstrated antiplasmodial activity with a moderate level according to Gessler criteria. Both of endophytic fungi have a great potential to be further investigated. © 2016, International Journal of Pharmaceutical and Clinical Research. All rights reserved.
Rumpun Ilmu
Biologi Farmasi
Bahasa Asli/Original Language
English
Level
Internasional
Status
Published
