Antioxidant activity assay of extracts and active fractions of kasturifruit (Mangifera casturi Kosterm.) using 1, 1-diphenyl-2-picryl hydrazyl method
Penulis/Author
Prof. Dr. apt. Subagus Wahyuono, M.Sc. (2); Prof. Dr. apt. Erna Prawita Setyowati, M.Si. (3); Prof. Dr. apt. Sugeng Riyanto, MS. (4); Dr. Agustinus Yuswanto, SU., Apt. (5)
Tanggal/Date
2014
Kata Kunci/Keyword
Abstrak/Abstract
In-vitro antioxidant activity assay was conducted on methanolic extracts and its
fractions (n-hexane fraction, ethylacetate fraction, and methanol fraction) of
Mangifera casturi fruit using DPPH (1,1-diphenyl-2- picrylhydrazyl) method. The
assay began by observing the chromatographic profile and phytochemical constituent
analysis. The analysis showed that methanolic extracts of M. casturi fruit and its
fractions contained compounds of terpenoid and polyphenol groups. Methanolic
extracts of M. casturi had antioxidant activity with an IC50 value of 112.4?g/ml, while
n-hexane, ethylacetate, and methanolic fractions had IC50 values of 193.0?g/ml,
6.0?g/ml, and 538.9?g/ml respectively.
