| Abstrak/Abstract |
Oxidative stress, the excessive presence of reactive oxygen species (ROS), is suggested as
a basal cause of aging as well as various degenerative and chronic diseases in human.
Antioxidants are believed to play a very vital role in the body defense system against
ROS. Plant-based antioxidants with their prominence have gained tremendous worldwide
interest nowadays. Baccaurea racemosa and Macaranga subpeltata are among
ethnomedical used plants for liver diseases medication which have potential source as
natural antioxidants. The study aimed to evaluate the antioxidant activities of the
methanolic crude extract (CE) and their fractions of the plant’s leaves. Maceration was
performed to obtain CE, which then subjected to fractionation using n-hexane,
dichloromethane, chloroform, ethyl acetate, and ethanol to obtain fractions of hexane
fraction (HF), dichloromethane (DF), chloroform (CF), ethyl acetate (EAF), and ethanol
fractions (EF), respectively. The CE and all fractions included water fraction (WF) and
residue (R) were examined for its total phenolic contents, total flavonoid contents, and
antioxidant activities using various in vitro assay. In general, EAF demonstrated as the
best solvent for the extracting phenolic compounds with higher antioxidant activity. The
CE and its fractions of M. subpeltata contained higher of TPC and TFC, also
demonstrated higher antioxidant capacity, than that B. racemosa. The phenolics
compounds were responsible for the antiradical properties. The EAF of M. subpeltata was
scavenging radicals better than that of L-(+)ascorbic acid as a positive control. The high
antioxidant activities and phenolics contents make both the plant extracts to be developed
as a food supplement. |
