Antimigration Activity of an Ethylacetate Fraction of Zanthoxylum acanthopodium DC. Fruits in 4T1 Breast Cancer Cells
Penulis/Author
Urip Harahap (1); Dr. dr. Lambok Siahaan MKT, Sp.ParK, Sp.KKLP (2); Panal Sitorus (3); dr. Nur Arfian, Ph.D. (4); Denny Satria (5)
Tanggal/Date
2018
Kata Kunci/Keyword
Abstrak/Abstract
Objective: This study was carried out to investigate the antimigration activity of Zanthoxylum acanthopodium DC.in the 4T1 breast cancer cell line. Methods: Zanthoxylum acanthopodium DC. fruit powder was extracted by maceration method with n-hexane and ethylacetate solvents. Cytotoxicity and proliferation were assessed using the MTT method and the cell cycle by flow cytometry. In addition, wound healing assays were conducted by a microscopic method, and expression of COX-2 and VEGFR-2 were determined using qRT-PCR. Results: The IC50 of the ethylacetate fraction (EAF) was 48.1 ± 1.06 ?g/mL. The EAE at a concentration 10 ?g/mL with viable cells was 62.3 ± 0.28?ter 72 h incubation, with accumulation in the G2-M phase, inhibition of cell migration in the wound healing assay, and decrease in expression of COX-2 (0.62 ± 0.01) and VEGFR-2 (0.39 ± 0.003). Conclusion: The results reveal that an ethylacetate fraction of Zanthoxylum acanthopodium DC. fruits provides effective antimigration effects. Further studies are now planned to assess the potential of the ethylacetate fraction to inhibit angiogenesis in breast cancer and
determine underlying mechanisms.
Rumpun Ilmu
Ilmu Kedokteran Dasar & Biomedis
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Antimigration actiity of an ethylacetate fraction.pdf
[PAK] Full Dokumen
2
Check similarity_Antimigration Activity of an Ethylacetate Fraction of Zanthoxylum acanthopodium DC_ Fruits in 4T1 Breast Cancer Cells.pdf
