Antimicrobial and Cytotoxic Activities of Endophytic Fungi Isolated from Artemisia annua L
Penulis/Author
Prof. Dr. Puji Astuti, S.Si., M.Sc., Apt. (1); Prof. Dr. Drs. Wahyono, SU., Apt. (2); Prof. dr. Titik Nuryastuti, M.Si., Ph.D., Sp.MK(K). (3); Dr. apt. Indah Purwantini, S.Si., M.Si. (4); Purwanto, M.Sc., Ph.D., Apt. (5)
Tanggal/Date
2014
Kata Kunci/Keyword
Abstrak/Abstract
This study was aimed to investigate antimicrobial and cytotoxic effects of endophytic fungi isolated from
A. annua L. The ethyl acetate extracts were obtained by liquid-liquid partition of fermentation broth of
endophytes followed by evaporation. Antimicrobial assay was determined by disc diffusion method. Cytotoxicty
assay was conducted against T47D, WiDr and Vero using MTT assay. Data generated were used to determine
IC50. While DP2 extract did not show any inhibition against all tested bacteria, DP6 extract at 200 ?g inhibited
the growth of B. subtilis, P. aeruginosa and S. thypi with the highest inhibition against B. subtilis. Similarly, E2
extract inhibited the growth of B. subtilis, E. coli and S. thypi, with the highest inhibition against B. subtilis. The
three extracts (DP2, DP6 and E2) were found to be cytotoxic against T47D and WiDr with E2 extract to be the
most cytotoxic. These compounds were more selective against T47D cell line with IC50 of 91.7, 74.8 and 12.0
?g/mL, respectively. The IC50 against Vero cell line are 444.4, 140.3 and 87.7 ?g/mL, respectively
Rumpun Ilmu
Biologi Farmasi
Bahasa Asli/Original Language
English
Level
Internasional
Status
Dokumen Karya
No
Judul
Tipe Dokumen
Aksi
1
Antimicrobial and Cytotoxic Activities of Endophytic Fungi Isolated from Artemisia annua L.pdf
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2
Antimicrobial and Cytotoxic Activities of Endophytic Fungi Isolated.pdf
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3
19-Astuti et al 2014-JAPS- proof of correspondence-revisi.pdf
