Antigen Ekskretori-Sekretori Cacing Jantung (Dirofilaria immitis) Jantan dan Betina yang Berpotensi Sebagai Marka Diagnosis
Penulis/Author
Dr. I Gusti Made Ngurah Budiana, S.Si., M.Si. (1); Prof. Dr. drh. Ida Tjahajati, MP. (2); Prof. drh. Widya Asmara, SU., Ph.D. (3); Prof. Dr. drh. Raden Wisnu Nurcahyo (4)
Tanggal/Date
5 2016
Kata Kunci/Keyword
Abstrak/Abstract
Heart worm (
Dirofilaria immitis
) is the causative agent of a serious parasitic disease in dogs.
Dirofilariasis is generally diagnosed by microfilariae examination and specific antigen testing. Microfilariae
examination has low sensitivity due to occult infections. The available antigen test at this time is able to
detect circulating antigens secreted by adult female worms only. The aim of the present study was to
identify male (MES) and female (FES) heart worms excretory-secretory antigens which have the potential
as a diagnostic targets. Identification of antigen was done by sodium dodecyl sulphate polyacrylamide gel
electrophoresis (SDS-PAGE) and Western Blotting analysis. The results of this study indicated that there
were differences between the MES and the FES profiles. The results showed 12 bands in MES (14–118
kDa) and 18 bands in FES (10–205 kDa). Protein with a molecular weight of 59 kDa has the potential as
diagnostic markers of dirofilariasis.
