| Abstrak/Abstract |
epressionisapsychiatricdisorderthataffectsmoodandphysicalhealthand contributesgreatlytotheglobalburdenofdisease.Herbal remediescanbeusedasanalternativetothetreatmentofdepression.Thepurposeofthisstudyistodeterminetheactivityof80%ethanol extractofbittermelonleaves(MomordicacharantiaLinn)asanantidepressantandβ-sitosterolcompoundwhichisamarkercompound in bitter melon leaf extract. In this study, β-sitosterol compounds in bitter melon leaf extract were identifiedqualitatively using Fourier TransformInfraRed(FTIR)andinvivo antidepressantactivitytests.Thein-vivotestused30micewithaweightof20-30gramswhich wererandomlydividedinto6groups,namelythenormalgroup(notreceivingtreatment),thenegativecontrolgroup(Na-CMC1%),the positive control group (fluoxetine 2.5 mg/kg), the ꞵ-sitosterol group (ꞵ-sitosterol30 mg/kg), the bitter melon leaf extract group dose 1 (200mg/kg),andthegroupofbittermelonleafextractdose2(400mg/kg).ThemicereceivedstressorsusingtheChronicUnpredictable Mild Stress (CUMS) method for 28 days. On the 15th day after the mice received CUMS, the mice were exposed to test extracts and drugs for14 daysaccording to the treatment group. Weight measurements were taken on day 1 andon day 29. Thebehavioraltestused the Forced Swimming Test (FST) and Novel Suppressed Feeding (NSF) with feeding time parameters, and the immobility time was carried out on the 29th day, in addition to that the number of necrosis in gastric and the number of hippocampal pyramidal cells were also measured. The results showed that at all absorption peaks in the FTIR of bitter melon leaf extract, there were -OH, C-H, C=C, -CH2-,C-Ogroupswhicharethecharacteristicpeaksoftheꞵ-sitosterolcompound.Inweightmeasurement,bittermelonleafextracthad antidepressant activity by not causing significant weight changes compared to the normal group (P>0.05). The β-sitosterol and bitter melon leaf extract group dose 2 had antidepressant activity by maintaining appetite which was shown with a higher feeding time value compared to the negative group (P<0>0.05)hadantidepressantactivityvaluesbyloweringdespairindicated bysmallerimmobilitytimevalues,loweringthenumberofnecrosisingastriclowerandprotectingthehippocampalpyramidal cellcount higher. Conclusion: 80% ethanol extract of bitter melon leaves contains active compounds β-sitosterol and has activity as an antidepressant by not causing weight changes, maintaining appetite, lowering despair, protecting gastric from necrosis and protecting hippocampal pyramidal cells. |
