Antibacterial and Antibiofilm Properties of Kulim Bark (Scorodocarpus borneensis Becc.) Hydrogel on Polymicrobial Biofilms
Penulis/Author
Dr. Sylvia Utami Tunjung Pratiwi, S.Si., M.Si. (2); Dr.rer.nat. apt. Yosi Bayu Murti, S.Si., M.Si. (3); Dr. Ema Damayanti, S.Si., M.Biotech (4)
Tanggal/Date
28 2025
Kata Kunci/Keyword
Abstrak/Abstract
Objectives: The study aimed to analyze the antibacterial and antibiofilm activity of hydrogels from Kulim (Scorodocarpus borneensis Becc.) stem bark extract and fraction against
polymicrobial biofilms.
Methods: The stem bark extract and fraction of Kulim (Scorodocarpus borneensis Becc.)
were formulated into a hydrogel (tween 80:glycerin). Antibacterial and antibiofilm activities
were assessed using a microdilution test against polymicrobial growth and polymicrobial
biofilms, while acute toxicity was evaluated using wader pari (Rasbora lateristriata) embryos.
Results: Kulim extract hydrogel (4 μg/µL) showed 69.16% antibacterial activity and
61.25% biofilm inhibition, with 72.58% and 82.86% biofilm degradation at 24 and 48
hours, respectively. The fraction-based hydrogel (0.22 μg/µL) had 58.10% antibacterial
and biofilm inhibition activity, with 81.14% and 79.64?gradation at 24 and 48 hours.
MIC50 and MBIC50 values were 2.42 μg/µL (extract) and 0.15 μg/µL (fraction) as well as
2.83 μg/µL and 0.19 μg/µL, respectively. Based on observation, MBEC50 values were 1.09
μg/µL and 0.57 μg/µL (24 h), as well as 0.70 μg/µL and 0.03 μg/µL (48 h), respectively,
while the extract’s LC50 was 0.143 mg/mL.
Conclusion: The hydrogel made from Kulim stem bark extract exhibited stronger antibacterial and antibiofilm activities than the ethyl acetate fraction against polymicrobial.
