Antibacterial activity and characterization of Annona muricata Linn leaf extract-nanoparticles against Escherichia coli FNCC-0091 and Salmonella typhimurium FNCC-0050
The objective of this study was to evaluate the characterization and inhibitory effects
of Annona muricata Linn leaf extract loaded by chitosan nanoparticles on the growth of
Escherichia coli FNCC-0091 and Salmonella typhimurium FNCC-0050. The chitosan-sodium
tripolyphosphate-nano particles (CS-TPP-NPs) were prepared by ionic cross-linking of CSTPP and were characterized physico-chemically (particle size, zeta potential) and
morphologically. Antibacterial activity of the CS-TPP-NPs-AmE was performed by well
diffusion method. The optimum formula for CS-TPP-NPs was analyzed using centrifugation at
3,500 rpm for 30 min. A wide range, 1:1:0.02 to 1:1:0.01 of CS:AmE:TPP ratios were
evaluated with constant CS. Data obtained for bacterial activities were statistically analysed
using one-way analysis of variance (ANOVA). The means between groups were separated
with Duncan post-hoc test. The result showed that the optimum formula for CS-TPP-NPs of
AmE was 1:1:0.01. The optimum formula of CS-TPP-NPs-AmE was characterized by particle
size, zeta potential, and morphology. Results showed that the particle size of CS-TPP-NPsAmE was 234.00 ± 21.5 nm with spherical shape and positive charges on their surface with the
zeta potentials 34.77 ± 4.97 mV. CS-TPP-NPs of AmE in the ratio 1:1:0.01 has efficient
antibacterial activities against E. coli FNCC-0091 and S. typhimurium FNCC-0050
