| Abstrak/Abstract |
The antiatherosclerosis effect of purified Andrographis paniculata extract (PAE) was studied on atherosclerotic Wistar male albino rats. A fat-rich diet containing 10% yolk, 15% fat of pig, 1% calcium, 20,000 IU vitamin D3 was administered to albino male wistar rats for 60 days to induce atherosclerosis. The two groups atherosclerosis rats were treated orally with PAE for 30 days at doses of 24 and 70 mg/kg body weight, respectively. Its lipid level in blood was measured at before and the last of the PAE treatments. At the end of the PAE treatments, the rats were sacrificed and the morphology of their aorta was observed under a microscope. A TLC-densitometer was used to analyze the andrographolide content in the PAE. The results showed that andrographolide was found in the PAE as it produced Rf value of 0.5 which was similar to andrographolide refference standard. The fat-rich diet supplemented with vitamin D3 and calcium induced dyslipidemia and atherosclerosis in rats. This was indicated by accumulation of fat plaques or calcification on the wall of their arteries. The PAE can become an antidyslipidemia by lowering total cholesterol, triglyceride and LDL-C and also increasing HDL-C in blood (p< 0.05). The PAE dose of 70 mg/kg body weight, removed lipid plaques significantly until the same level observed in normal conditions). The andrographolide content of the PAE might be responsible for the removal of lipid plaques on the wall of rat arteries. |
