| Abstrak/Abstract |
The antiatherosclerosis effect of purified Andrographis paniculata
extract (PAE) was s tudied on atherosclerotic Wistar male albino rats. A
fat-rich diet containing 10% yolk, 15% fat of pig, 1% calcium, 20,000 IU
vitamin D3 was administrated to albino male wistar rats for 60 days to
induce atherosclerosis. The two groups atherosclerosis rats were
treated orally with PAE for 30 days at doses of 24 and 70 mg/kg body
weight, respectively. Its lipid level in blood was measured at before and
the last of the PAE treatments. At the end of the PAE treatments, the
rats were sacrificed and the morphology of their aorta was observed
under a microscope. A TLC-densitometer was used to analyze the
andrographolide content in the PAE. The results showed that
andrographolide was found in the PAE as it produced Rf value of 0.5
which was similar to andrographolide refference standard. The fat-rich
diet supplemented with vitamin D3 and calcium induced dyslipidemia
and atherosclerosis in rats. This was indicated by accumulation of fat
plaques or calcification on the wall of their arteries. The PAE can
become an antidyslipidemia by lowering total cholesterol, triglyceride
and LDL-C and also increasing HDL-C in blood (p< 0.05). The PAE dose
of 70 mg/kg body weight, removed lipid plaques significantly until the
same level observed in normal conditions). The andrographolide
content of the PAE might be responsible for the removal of lipid
plaques on the wall of rat arteries. |
