ACADSTAFF UGM
| Judul/Title | Anti-Osteoporosis Potencies of Zingiber officinale Rosc. Rhizome Water Extract and DFA III Produced from Dahlia spp. L.: In Vivo and In Vitro Studies |
| Penulis/Author | Dr. apt. Muthi' Ikawati, M.Sc. (1); Yogi Ertanto (2); Een Sri Endah (3); Sri Pudjiraharti (4); Prof. Dr. apt. Edy Meiyanto, M.Si. (5); Dr. Riris Istighfari Jenie, M.Si., Apt. (6) |
| Tanggal/Date | 2022 |
| Kata Kunci/Keyword | |
| Abstrak/Abstract | Background: Zingiber officinale Rosc. is estrogenic and thus can be developed as an anti-osteoporosis. Difructose anhydride III (DFA III), a functional oligosaccharide, possesses anti-osteoporosis potencies. This study aimed to investigate the anti-osteoporosis activity of ginger rhizome water extract (GE) and DFA III from dahlia tubers in ovariectomized (OVX) rat models and to determine their anti-osteoclastogenic effect in vitro. Methods: This study was conducted using 30 female rats divided into five treatment groups (sham-operated; OVX; OVX + GE; OVX + DFA III; and OVX + GE + DFA III). Blood sampling was carried out at the beginning and end of treatments. Femur bones were isolated after daily 14-day treatments, measured for density, and processed for histological staining. RAW 264.7 cells were induced by osteoclast differentiation factor. A cell viability assay was employed to determine the cytotoxicity of DFA III and GE. The inhibition of osteoclastogenesis was investigated by tartrate-resistant acid phosphatase staining. Results: All groups showed no difference in body weight elevation and serum lipid profiles. The GE and DFA III caused no effect on the bone density. However, the GE or DFA III groups showed higher osteoblast numbers compared with the control groups. A significantly less osteoclast was found in the GE + DFA III group. The GE and DFA III showed no toxicity on RAW 264.7 cells. DFA III had a weak osteoclastogenesis inhibitory activity compared with GE, which showed strong inhibitory effects on the post stimulation osteoclastogenesis model. The combination of GE and DFA III was synergistic in reducing the osteoclastogenesis confluency in RAW 264.7 cells. Conclusion: The data support our hypothesis that GE and DFA III can decrease the risk of osteoporosis by osteoclastogenesis inhibition. |
| Rumpun Ilmu | Hidrometeorologi Departemen Geografi Pembangunan |
| Bahasa Asli/Original Language | English |
| Level | Internasional |
| Status |